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Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based  Applications, Genomic Integrations and Combinatorial Libraries | ACS  Synthetic Biology
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries | ACS Synthetic Biology

Plasmid Construction: Building the Future: Plasmid Construction with CCDB -  FasterCapital
Plasmid Construction: Building the Future: Plasmid Construction with CCDB - FasterCapital

Team:Leiden/Experiments - 2021.igem.org
Team:Leiden/Experiments - 2021.igem.org

Addgene: pLIX403-iRFP-ccdB-Puro
Addgene: pLIX403-iRFP-ccdB-Puro

Construction and schematic of plasmid. a pEn-Chimera-ccdB. A cassette... |  Download Scientific Diagram
Construction and schematic of plasmid. a pEn-Chimera-ccdB. A cassette... | Download Scientific Diagram

ZeBRα a universal, multi-fragment DNA-assembly-system with minimal hands-on  time requirement | Scientific Reports
ZeBRα a universal, multi-fragment DNA-assembly-system with minimal hands-on time requirement | Scientific Reports

The FX cloning principle. (A) Cloning of open reading frames (ORFs).... |  Download Scientific Diagram
The FX cloning principle. (A) Cloning of open reading frames (ORFs).... | Download Scientific Diagram

Addgene: pRosa26 R1-ccdB-R2 RexNeo PI-SceI
Addgene: pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Advantages Of Using Ccdb For Dna Barcoding - FasterCapital
Advantages Of Using Ccdb For Dna Barcoding - FasterCapital

Direct cloning and heterologous expression of the salinomycin biosynthetic  gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor  A3(2) | Scientific Reports
Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2) | Scientific Reports

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

A versatile toolbox for knock-in gene targeting based on the Multisite  Gateway technology | PLOS ONE
A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology | PLOS ONE

A unified multi-kingdom Golden Gate cloning platform | Scientific Reports
A unified multi-kingdom Golden Gate cloning platform | Scientific Reports

Pathogens | Free Full-Text | Antibiotic-Free Selection in Biotherapeutics:  Now and Forever
Pathogens | Free Full-Text | Antibiotic-Free Selection in Biotherapeutics: Now and Forever

Plasmids and functional tests. (A) p15A-ccdB-amp contains the ccdB-amp... |  Download Scientific Diagram
Plasmids and functional tests. (A) p15A-ccdB-amp contains the ccdB-amp... | Download Scientific Diagram

Directed evolution of CRISPR-Cas9 to increase its specificity | Nature  Communications
Directed evolution of CRISPR-Cas9 to increase its specificity | Nature Communications

Role of CCDB - FasterCapital
Role of CCDB - FasterCapital

Addgene: p2R3z-BASI-ccdB-BSAI
Addgene: p2R3z-BASI-ccdB-BSAI

Principle of ccdB-gene replacement strategy. The schematic... | Download  Scientific Diagram
Principle of ccdB-gene replacement strategy. The schematic... | Download Scientific Diagram

Selection Pressures: Alternatives to Antibiotics in Molecular Cloning
Selection Pressures: Alternatives to Antibiotics in Molecular Cloning

Addgene: MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1
Addgene: MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

Gateway Destination vectors constructed in this study. (A) Yeast... |  Download Scientific Diagram
Gateway Destination vectors constructed in this study. (A) Yeast... | Download Scientific Diagram

Addgene: pBCN42-R4R3
Addgene: pBCN42-R4R3

Principle of ccdB-gene replacement strategy. The schematic... | Download  Scientific Diagram
Principle of ccdB-gene replacement strategy. The schematic... | Download Scientific Diagram